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    題名: 靜磁場對於自然殺手細胞之毒殺能力的影響
    The Influence of Static Magnetic Fields on cytotoxicity of Natural Killer Cells
    作者: 蘇怡彩
    Su, Yi-Tsai
    貢獻者: 生醫材料暨組織工程研究所
    黃豪銘
    關鍵詞: 自然殺手細胞;靜磁場;人類血癌細胞株
    K562Natural Killer Cells;Static Magnetic Fields;K562
    日期: 2012-12-17
    上傳時間: 2018-10-03 17:33:50 (UTC+8)
    摘要: 根據行政院衛生署統計資料顯示，癌症已經蟬聯28年十大死因的榜首，有鑑於此尋找一種有效且不會對正常的細胞產生不良影響的治療方式愈來愈重要。基於此原因，免疫細胞療法逐漸地受到重視。自然殺手細胞在人體的免疫系統中屬於先天免疫，在體內可以主動的去辨識癌細胞，並可以藉由分泌細胞激素對於癌細胞進行毒殺作用。
    由於根據先前的研究指出，靜磁場會改變細胞膜雙層磷脂質結構而對細胞膜表面造成影響，也會進一步使細胞膜表面的受體和配體間的親和力下降。而自然殺手細胞毒殺細胞的原理是藉由自然殺手細胞與目標細胞之間的訊號的平衡來達到毒殺目標細胞（癌細胞、被病毒感染的細胞）的目的。實驗方法上，先將NK-92MI細胞培養在4000高斯的銣鐵硼磁鐵上，之後以MTT法分析NK-92MI細胞數量上的改變。之後將NK-92MI細胞與目標細胞K562共同培養4小時後以MTT法測定存活的K562細胞比率並藉此推算NK-92MI細胞的毒殺能力。
    實驗結果顯示在未預先暴露靜磁場的情形下，效能細胞與目標細胞比（E/T ratio）為1:5、1:2、1:1、1:0.5的情況下暴露於靜磁場的組別其毒殺能力與未暴露組均沒有差異性。而在有預暴靜磁場的組別在效能細胞與目標細胞比為1:1的情況下，其毒殺能力均顯著高於對照組（P＜0.05）。本研究探討靜磁場對自然殺手細胞生長及毒殺能力的影響及訊息傳遞路徑。由實驗結果證明靜磁場會透過MAPK pathway來影響自然殺手細胞NK-92MI的生長速度。根據此一結果可以推論，靜磁場會藉由影響NK-92MI細胞的數目並進一步影響自然殺手細胞的毒殺能力。
    According to the Department of Health statistics show that cancer has been reelected to the top of the ten leading causes of death for 28 years. Therefore searching for an no effective treatment without side effect become more and more important. The previous studies show that the static magnetic field will change the cell membrane bilayer of phospholipid structure, and it will be further decreased affinity cell surface receptors and ligands. The mechanism of the cytotoxicity of NK cells is by the balance of the signals between the natural killer cells and target cells.
    In this study, NK-92MI cells were cultured with 4000 Gauss SMF. And we use MTT assay to analysis the changes in the number of NK-92MI, and the cytotoxicity of NK-92MI cells is measured by target cells`s survival ratio by MTT assay.
    The results show that the cytotoxicity of NK-92MI is no significant differences between control and exposed with static magnetic field at E/T ratio=1:5,1:2,1:1,1:0.5. When the NK-92MI cells pre-exposed with static magnetic field at E/T =1:1 , the cytotoxicity of static magnetic field exposure groups is significantly higher than that in control group (P <0.05). The experimental results prove that the static magnetic field to affect the growth rate of natural killer cells, NK-92MI through MAPK pathway.This result can be inferred that the static magnetic field will further impact by affecting the number of NK-92MI cells and natural killer cell cytotoxicity.
    描述: 碩士
    指導教授-黃豪銘
    委員-張維仁
    委員-蕭裕源
    資料類型: thesis
    顯示於類別:[生醫材料暨組織工程研究所] 博碩士論文

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