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    請使用永久網址來引用或連結此文件: http://libir.tmu.edu.tw/handle/987654321/44708


    題名: Rapid Induction of High-frequency MTL Homozygosis and Microbiological Polymorphism in Candida albicans by Fluconazole
    作者: Tsong-Yih Ou;Fang-Mo Chang;Ming-Li Chou;Wei-Ning Cheng;Kai-Cheng Lee;Che-
    關鍵詞: Candida albicans、fluconazole、mating type like gene、homozygosis
    日期: 2011-08
    上傳時間: 2012-01-19 09:11:07 (UTC+8)
    摘要: Candida α lbicans is most common fungal pathogen of opportunistic infections. C.
    albicans was previously, noted as a diploid, apparently asexual fungus. Morphological and
    genetic studies had found mating type-like locus (MTL a/α) and demonstrated its relations to
    morphological switching, virulence, and f1uconazole-resistance. A parasexual cycle was found
    between opposite MTL homozygotes. However, C. albicans was found with MTL
    homozygous genotype in only around 3.2 % of clinical s仕ains ， and MTL heterozygotes had a
    low 企equency (about 10勻of white-opaque (刺刀0) switch to be MTL homozygotes in nature.
    In the present study, reference C. αlbicans strain SC5314 was used for f1uconazole-inducing
    assay to harvest 35 first-generation survival daughter strains from 70 colonies picked 企om
    f1uconazole inhibitory zone. Further separation with spreading culture and micromanipulator
    methods was further employed to gain second- and third-generation strains with accordance to
    first-generation daughter strains. PCR analysis based on MTL a1 ， α1 and cf2 was employed to
    demonstrate MTL genotypes of these isolates. Microscopic observation and f1owcytometry
    assay were employed for cell/colony morphology and DNA content analysis on progeny
    SC5314 and its daughter strains. High 企equency of MTL gene loss (24 of 35, 68.57 %) and
    homozygotes (16 of 鈣， 45.71 % ) were found in f1uconazole-inducing survival daughter
    strains with accordance to first-generation daughter strains. Polymorphism of ceIVcolony
    morphology and decreasing DNA content were also found in these f1uconazole-inducing
    daughter strains. Fluconazole treatment not only inhibited the growth of C. α lbicans but also
    altered phenotypic characteristics in ceIVcolony morphology as well as induced rapid highfrequency
    MTL gene loss and homozygosity in f1uconazole inhibition zone. The DNA content
    of these f1uconazole-inducing daughter strains were also obviously reduced with comparison
    to their progeny SC5314 suggesting a possibility of chromosome loss and DNA
    rearrangement during the f1uconazole treatment to C. albicαns.
    關聯: Asian Mycological Congress
    顯示於類別:[內科部] 期刊論文

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