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    題名: Establishment of a young mouse model and identification of an allelic variation of zmpB in complicated pneumonia caused by Streptococcus pneumoniae
    作者: Yu-Chia Hsieh;Po-Nien Tsao;Chi-Long Chen;Tzu-Lung Lin;Wen-Sen Lee;Pei-Lan Shao;Chin-Yun Lee;Po-Ren Hsueh;Li-Min Huang;Jin-Town Wang
    關鍵詞: complicated pneumonia,microarray,Streptococcuspneumoniae,allelic variation
    日期: 2008
    上傳時間: 2011-10-12 13:45:23 (UTC+8)
    摘要: Objective: Complicated pneumonia, including necrotizing pneumonia,
    lung abscess, and empyema, caused by Streptococcus pneumoniae
    in children has been increasing. We thus determined to
    investigate its virulence in an animal model and to identify virulence
    factors of S. pneumoniae.
    Design: Prospective, randomized, controlled animal study.
    Setting: University medical laboratory.
    Subjects: Male Balb/c-strain mice, 3 wks old.
    Interventions: We used a young mouse model to monitor
    bacterial virulence and a microarray to compare gene expression
    between S. pneumoniae from children with complicated and noncomplicated
    pneumonia. Deletion and complementation of a candidate
    gene were performed to study its role on the virulence of
    S. pneumoniae.
    Measurements and Main Results: A model of complicated
    pneumonia in young mice infected with strains of S. pneumoniae
    from children with complicated pneumonia was established. Using
    a microarray analysis, differences in zinc metalloprotease B
    (zmpB) RNA hybridization between two strains from children with
    complicated pneumonia (NTUH-p28 and NTUH-p15) and a strain
    (NTUH-p3) from a child with pneumococcal lobar pneumonia were
    found. Confirmatory assays revealed the signal differences were
    due to sequence variation in the zmpB gene. Infection with the
    zmpB deletion mutant of NTUH-p15 showed a significant decrease
    in the severity of pneumonia and no destructive lung injury. The
    zmpB complementation strain of NTUH-p15 significantly restored
    the level of inflammation and caused lung necrosis. For studying
    the effect of allelic variation of zmpB on the virulence of S.
    pneumoniae, we added zmpB of NTUH-p15 in the zmpB deletion
    mutant of NTUH-p3, which resulted in a higher bacterial burden
    than that in wild-type NTUH-p3.
    Conclusions: A young mouse model is established for complicated
    pneumococcal pneumonia. This model proved that allelic
    variation of zmpB affects the virulence of S. pneumoniae. (Crit
    Care Med 2008; 36:1248–1255)
    關聯: Critical Care Medicine 36(4): 3226-3232.
    顯示於類別:[內科部] 期刊論文

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