Oxidative stress status in sperm with declined motility.

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Taipei Medical University Institutional Repository > 醫學科技學院 > 醫學檢驗暨生物技術學系所 > 期刊論文 > Item 987654321/3778

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題名:	Oxidative stress status in sperm with declined motility.
作者:	高淑慧 Kao SH;Chao HT;Chen HW;Hwang TIS;Liao TL;Wei YH
貢獻者:	醫學檢驗暨生物技術學系
日期:	2008
上傳時間:	2009-08-25 10:38:47 (UTC+8)
摘要:	OBJECTIVE: To investigate the causal role of oxidative-stress status on human sperm motility. DESIGN: To demonstrate that sperm with higher oxidative damage have a lower antioxidant capacity. SETTING: University hospital infertility center. PATIENT(S): Seventy-eight semen samples were obtained from 35 healthy donors who had normal semen characteristics and from 43 infertile or subfertile males. INTERVENTION(S): The levels of oxidative damage (8-hydroxy-2'-deoxyguanosine [8-OHdG] and lipid peroxides) and antioxidants (retinol, alpha-tocopherol, ascorbate, and protein thiols) in the spermatozoa and/or seminal plasma were measured. MAIN OUTCOME MEASURE(S): We analyzed the specific content of 8-OHdG and lipid peroxides by using high-performance liquid chromatography (HPLC)-electrochemical detection and HPLC-fluorescence analysis, respectively. Retinol and alpha-tocopherol were analyzed by using an HPLC system, whereas ascorbate and protein thiols were determined by using spectrophotometry. 8-Hydroxy-2'-deoxyguanosine was visualized by immunofluorescent staining with an anti-8-OHdG antibody that was conjugated with fluorescein isothiocyanate conjugate. Lipid peroxides in spermatozoa were stained with a fluorescent dye, C11-BODIPY(581/591). RESULT(S): Statistically significant negative correlations were revealed between sperm motility and 8-OHdG and between motility and lipid peroxides. Statistically significant positive correlations were found between sperm motility and the levels of retinol, alpha-tocopherol, ascorbate, and protein thiols of seminal plasma. 8-Hydroxy-2'-deoxyguanosine and lipid peroxides in spermatozoa were found to be present mostly in mitochondria. CONCLUSION(S): Oxidative stress and oxidative damage were increased significantly in spermatozoa with declined motility, and the antioxidant capacities in the spermatozoa and seminal plasma were lower in males who had infertility or subfertility.
關聯:	Fertil Steril.(89):1183-1190.
資料類型:	article
顯示於類別:	[醫學檢驗暨生物技術學系所] 期刊論文

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