Induction of platelet aggregation by SAS tongue cancer cells: a mechanism of hematogenous metastasis

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Taipei Medical University Institutional Repository > 醫學科技學院 > 醫學檢驗暨生物技術學系所 > 期刊論文 > Item 987654321/3764

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題名:	Induction of platelet aggregation by SAS tongue cancer cells: a mechanism of hematogenous metastasis
作者:	何元順 Mei-Chi ChangChiu-Po Chan;Yuan-Soon Ho;Jang-Jaer- Lee;Po-Shuen Lin;Bor-Ru Lin;Ya-Ling Huang;Liang-Jiunn Hahn;Hung-
貢獻者:	醫學檢驗暨生物技術學系
日期:	2008
上傳時間:	2009-08-25 10:38:31 (UTC+8)
摘要:	BACKGROUND: Tongue cancer metastasis is mainly through blood stream and possibly associated with tumor cell-induced platelet aggregation (TCIPA). METHODS: Platelet aggregation was induced by different amounts of SAS tongue cancer cells with⁄without inhibitors and the latent period for induction of platelet aggregation was recorded. Gene expression was analyzed by reverse transcriptase-polymerase chain reaction. RESULTS: SAS cells (4 · 104 to 1 · 106 cells⁄ml) induced platelet aggregation in a cell density-dependent manner. The latent period for induction of platelet aggregation reduced from 11.3 min (2 · 105 cells⁄ml) to 0.9 min (5 · 105 cells⁄ml). The extent of platelet aggregation increased from 39% to 76% by 2 · 105 and 5 · 105 SAS cells. Pre-treatment of SAS cells with aspirin showed little effect on its induction of platelet aggregation. SAS cells expressed tissue factor (TF) mRNA and the SAS cells-induced TCIPA was inhibited by TF neutralization antibody (5–20 lg⁄ml), heparin (5–10 U⁄ml), Hirudin fragment 54–65 (50 lg⁄ml) and D-Phenylalanyl-L-prolyl-L-arginine chloromethyl ketone. But areca nut (AN, a betel quid component known to generate reactive oxygen species (ROS)) extract showed little effect on TF expression in SAS cells. Pretreatment with U73122 and 2-aminoethoxydiphenylborate inhibited SAS-induced TCIPA. Interestingly, catalase suppressed SAS cells-induced TCIPA, whereas AN extract enhanced this event. CONCLUSIONS: These results suggest that tongue cancer cells may induce TCIPA and enhance tumor metastasis. SAS-induced TCIPA is related to TF secretion, thrombin generation and associated with Phospholipase C-Inositol triphosphate signaling and ROS production. Betel quid chewing may potentially promote tongue cancer metastasis.
關聯:	Journal of Oral Pathology and Medicine.
資料類型:	article
顯示於類別:	[醫學檢驗暨生物技術學系所] 期刊論文

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