Human papillomavirus typing with a polymerase chain reaction-based genotyping array compared with type-specific PCR

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Title:	Human papillomavirus typing with a polymerase chain reaction-based genotyping array compared with type-specific PCR
Authors:	林景堉 Lin CY;Chao Angel;Chou;HH;Yang YC;Ho CM;Lin RW;Chang TC;Chiou JY;Chao FY;Wang KL;Chi
Contributors:	醫學檢驗暨生物技術學系
Keywords:	Human papillomavirus, Type-specific PCR, HPV Blot, Agreement
Date:	2008
Issue Date:	2009-08-25 10:38:24 (UTC+8)
Abstract:	Abstract Background: Type-specific persistence of human papillomavirus (HPV) infection can cause invasive cervical cancer. Objectives: To evaluate the efficacy of HPV detection and typing with a general polymerase chain reaction (PCR)-based genotyping array and to compare it with a type-specific PCR assay. Study design: Four hundred and thirty-three cervical samples were tested with a modified MY11/GP6+ PCR-based reverse-blot assay (EasyChip® HPV Blot; King Car, Taiwan [hereafter HPV Blot]) and with 20 genotypes of L1-type-specific PCR (HPV-6, -11, -16, -18, -31, -33, -35, -39, -45, -51, -52, -53, -56, -58, -59, -62, -66, -68, -70, and -71 [CP8061]). Results: The concordance of the two tests in determining HPV positivity was 96.8% (419/433), with a Cohen’s κ = 0.93 (95% CI: 0.90–0.97) and McNemar’s test of P = 1.0, which indicates excellent agreement. The overall concordance of the two tests in the identification of typespecific HPV was 91.0% (394/433). Sensitivity (90–100%), specificity (99.2–100%), and accuracy (98.6–100%) rates of HPV Blot against the gold standard were satisfactory for HPV-16, -18, -58, -33, -52, -39, -45, -31, -51, -70 while HPV-71 (63.6%) had suboptimal sensitivity. Though the κ values between the two tests for many individual genotypes could not be reliably calculated because of low positivity, the κ values for HPV-16, -52, and -58 were excellent (0.93, 0.96, and 0.95, respectively). Conclusion: The modified MY11/GP6+ PCR-based HPV Blot assay is accurate and sensitive for detection and genotyping of HPV in cervical swab samples.
Relation:	Journal of Clinical Virology.(42):361-367.
Data Type:	article
Appears in Collections:	[ ] Periodical Articles

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