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| 題名: | 兔尾草之成分探討 |
| 作者: | 洪筱喬 |
| 貢獻者: | 生藥學研究所 |
| 日期: | 2010 |
| 上傳時間: | 2010-10-21 09:23:16 (UTC+8) |
| 摘要: | 兔尾草（Uraria crinita（L.）Desv. ex DC.）為豆科（Fabaceae）兔尾草屬（Uraria）多年生草本亞灌木，廣泛分布於中國大陸南部、海南島、香港、緬甸、泰國、菲律賓、爪哇及台灣等地。兔尾草根部以 95% 乙醇於室溫下反覆萃取三次，經過濾、濃縮後，95% 乙醇萃取物分別以正己烷、乙酸乙酯、正丁醇進行分配萃取，進一步進行乙酸乙酯層及正丁醇層萃取物之分離與純化，經由各種管柱層析及再結晶等方法，共得到十一個化合物，由物理和光譜數據解析，發現其中一個為新化合物，屬於 flavonoid結構的化合物（3），其它分別為 flavonoids 結構的化合物（1）、（4）；phenolic acid 結構的化合物（2）；amino acid結構的化合物（5）；benzo-γ-pyrone glycosides結構的化合物（6）、（8）；purine類的化合物（7）；megastigmane glycoside結構的化合物（9）；neolignan glycoside結構的化合物（10）；pyrimidine類的化合物（11）。其中化合物（1）-（3）,（5）-（11）為首次自兔尾草所分離出，化合物（3）,（5）-（11）為首次自兔尾草屬所分離出。
Uraria crinita (L.) Desv. ex DC. (Fabaceae), is the perennial herb subshrub and widely distributed throughout southern China, Hainan Island, Hong Kong, Myanmar, Thailand, Philippines, Java and Taiwan. In the present study, the roots of U. crinita were extracted three times with 95% ethanol at room temperature. The 95% ethanol extract was filtered and concentrated under reduced pressure, then partitioned with a sequence of n-hexane, ethyl acetate (EtOAc) and n-butanol (n-BuOH). The EtOAc layer and n-BuOH layer were further isolated and purified. The constituents were obtained by a series of chromatographic analysis and recrystallization. Totally, eleven compounds including one new flavonoid (3), two known flavonoids (1) and (4), one phenolic acid (2), one amino acid (5), two benzo-γ-pyrone glycosides (6) and (8), one purine (7), one megastigmane glycoside (9), one neolignan glycoside (10) and one pyrimidine (11) were isolated and identified by spectral analysis. Among these compounds, compound (1) - (3), (5) - (11) were first isolated from U. crinita, compound (3), (5) - (11) were first isolated from the genus of Uraria. |
| 關聯: | 144頁 |
| 描述: | 摘要 i
Abstract ii
縮寫表 iii
第一章 緒論 1
一、 兔尾草介紹及其同屬植物文獻回顧 1
（一）兔尾草基本介紹 1
（二）兔尾草屬植物相關介紹 3
（三）兔尾草屬植物之近代活性研究及專利使用 5
（四）兔尾草屬植物之化學成分研究 7
二、 氧化壓力與巴金森氏症之介紹 16
（一）自由基（free radicals）與氧化壓力（oxidative stress） 16
（二）巴金森氏症 16
（三）氧化壓力與巴金森氏症之關係 20
三、 PC12細胞株及其相關介紹 21
（一）PC12細胞株簡介 21
（二）六羥基多巴胺（6-hydroxydopamine, 6-OHDA）之應用 21
（三）6-OHDA於PC12細胞的機轉 22
四、 研究動機 23
第二章 實驗材料與方法 24
一、 兔尾草之萃取與分離 24
（一）試藥及溶媒 24
（二）色層分析法材料 24
（三）儀器 25
（四）萃取及分離流程 26
（五）水解及單糖的組成分析 30
（六）各成分之物理數據 31
二、 兔尾草之神經保護實驗 41
（一）藥品與試劑 41
（二）儀器 41
（三）PC12細胞株及細胞培養液 41
（四）PC12細胞存活率試驗 41
（五）PC12細胞受6-OHDA損傷及活性成分保護之實驗 42
第三章 結果與討論 44
一、 兔尾草分離之化合物及其結構鑑定 44
（一）CP-1之結構解析 44
（二）CP-2之結構解析 48
（三）CP-3之結構解析 50
（四）CP-4之結構解析 53
（五）CP-5之結構解析 57
（六）CP-6之結構解析 60
（七）CP-7之結構解析 63
（八）CP-8之結構解析 66
（九）CP-9之結構解析 70
（十）CP-10之結構解析 74
（十一）CP-11之結構解析 78
二、 兔尾草之活性探討 80
三、 討論 81
第四章 結論 85
第五章 參考文獻 86
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