| 描述: | 致謝………………………………………………………………………1
目錄……………………………………………………………………2
表目錄…………………………………………………………………6
圖目錄…………………………………………………………………8
縮寫表…………………………………………………………………10
中文摘要………………………………………………………………12
英文摘要………………………………………………………………14
前言……………………………………………………………………16
試劑與儀器……………………………………………………………32
一、試藥與試劑…………………………………………………32
二、實驗儀器……………………………………………………35
三、試劑配製……………………………………………………37
四、植物材料……………………………………………………40
五、實驗動物……………………………………………………40
實驗方法………………………………………………………………41
一、 植物材料前處理與萃取…………………………………41
二、 氯化亞鐵(FeCl2)誘導大鼠肝臟粒腺體酯質過氧化之抑制能力…………………………………………………………42
(1)蛋白質定量……………………………………………42
(2)TEP標準曲線…………………………………………43
(3)YA-1於肝臟粒腺體酯質過氧化活性測定……………43
(4)抑制酯質過氧化計算公式……………………………44
三、四氯化碳(CCl4)誘導ICR小鼠急性肝損傷………………44
(1)YA-1萃取物對小鼠急性肝損傷之血清生化檢測……44
(2)YA-1萃取物對小鼠急性肝損傷之病理組織切片……45
(3)YA-1萃取物對肝臟粒線體DNA之保護……………45
四、總多酚、總黃酮與總黃烷醇含量測定……………………47
(1)總多酚含量測定(Total phenol)…………………………47
(2)總黃酮含量測定(Total flavonoid)………………………48
(3)總黃酮醇含量測定(Total flavonol)……………………48
五、自由基清除與抗氧化活性評估……………………………49
(1)清除DPPH自由基能力測定……………………………49
(2)螯合亞鐵離子能力測驗………………………………50
(3)清除超氧陰離子能力測定……………………………50
(4)抑制超氧陰離子能力測定……………………………51
(5)總還原能力測定………………………………………52
六、統計分析……………………………………………………53
實驗結果………………………………………………………………54
一、氯化亞鐵(FeCl2)誘導大鼠肝臟粒腺體酯質過氧化之抑制能力……………………………………………………………54
(1) MDA(TBA)2含量標準品TEP之標準曲線…………54
(2) 蛋白質定量標準品BSA之標準曲線………………55
(3) YA-1抑制肝臟粒線體脂質過氧化能力……………55
二、四氯化碳(CCl4)誘導ICR小鼠急性肝損傷之血清生化檢測……………………………………………………………59
三、四氯化碳(CCl4)誘導ICR小鼠急性肝損傷之病理組織切片……………………………………………………………61
四、YA-1萃取物對肝臟粒線體DNA之保護…………………65
五、總多酚含量…………………………………………………67
六、總黃酮含量…………………………………………………69
七、總黃酮醇含量………………………………………………71
八、清除DPPH自由基能力……………………………………72
九、螯合亞鐵離子能力…………………………………………75
十、清除超氧陰離子能力………………………………………78
十一、抑制超氧陰離子能力……………………………………81
十二、總還原能力………………………………………………83
討論……………………………………………………………………85
結論……………………………………………………………………89
參考文獻………………………………………………………………91
附錄…………………………………………………………..………100
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