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    題名: 健康成人攝取多樣化蔬果與體內氧化壓力之相關性
    The Association between Variety of Vegetables and Fruits Intake and Oxidative Stress in Healthy Adults
    作者: 黃千芬
    Huang Chien-Fen
    貢獻者: 保健營養學研究所
    劉珍芳
    關鍵詞: 碩士 指導教授-劉珍芳 委員-胡雪萍 委員-高美丁
    蔬果多樣化;蔬果攝取量;總多酚;氧化壓力
    the variety of vegetables and fruits;the intake of vegetables and fruits;polyphenols;oxidative stress
    日期: 2010
    上傳時間: 2010-10-20 12:49:53 (UTC+8)
    摘要: 本研究的目的為探討健康成人飲食中攝取多樣化蔬果與體內抗氧化狀態及氧化傷害指標之相關性。本橫斷面研究共招募 160 位年齡為 20~44 歲的健康成人,去除 21 位問卷填寫不完整或有個人因素的受試者,以 139 位之數據進行分析。受試者需填寫 76 項半定量蔬果頻率問卷並採集空腹血液,問卷內容為評估過去 1 個月內蔬果的攝取情形,蔬果項目依照植物性化學物質 (phytochemicals) 所呈現的顏色分為綠色、紅色、橘黃色、藍紫色與白色;血液分析包括測定抗氧化狀態與氧化傷害之指標。結果顯示,受試者每天平均總蔬果、蔬菜與水果的攝取量分別為 3.41、2.17 與 1.24 份。總蔬果的多樣化與總蔬果的攝取量呈顯著正相關。依所有受試者每天平均總蔬果的攝取量由低至高分成三組,以進行各數值的比較。血漿中總多酚、維生素 A 與維生素 E 濃度隨總蔬果的攝取量增加而增加 (p value for trend<0.05)。此外,綠色蔬菜和紅色水果的攝取量與血漿總多酚濃度呈顯著正相關、與血清 MDA (malondialdehyde) 濃度呈顯著負相關;紅色水果的攝取量與血漿總抗氧化力 (ferric reducing ability of plasma, FRAP) 呈顯著正相關;橘黃色蔬菜與橘黃色水果的攝取量與血漿維生素 A 濃度呈顯著正相關。然而,不同顏色蔬果的攝取量與血漿維生素 E 或血清 PC (protein carbonyl) 濃度皆無顯著相關。整體而言,本研究中成人每日蔬果的攝取量與 2005~2008 年國民營養健康狀況變遷調查的結果相近,且皆低於「每日飲食指南」建議量。然而,若能於飲食中增加總蔬果的攝取量,且以綠色蔬菜、紅色水果與橘黃色蔬果做為優先選擇的組合,對增加體內抗氧化能力與降低氧化傷害具有正面的幫助。
    This aim of this study was to investigate the association between the variety of vegetables and fruits intake on the biomarkers of antioxidant status and oxidative damage in healthy adults. The study design was under a cross-sectional model. One hundred and sixty healthy adults aged 20~44 years were recruited in this study, 21 subjects were excluded because of missing data or personal reasons. The variety and consumption of vegetables and fruits in the past month was assessed by using semi-quantitative food frequency questionnaire including 76 vegetables and fruits items. According to the colors of phytochemicals, the vegetable and fruits were grouped into 5 groups (red, blue-purple, orange-yellow, green and white). Fasting blood samples were collected for analysis of the antioxidant status and oxidative damage. The average daily intake of total vegetables and fruits, vegetables or fruits was 3.41, 2.17 or 1.24 servings, respectively. The consumption of vegetables and fruits were positively associated with variety. According to the consumption of total vegetables and fruits, subjects were divided into three groups. Higher total vegetables and fruits consumption was associated with higher vegetables and fruits consumption in different colors, and was also associated with higher plasma polyphenols, vitamin A and vitamin E (p<0.05). Multiple regression analysis showed that green vegetables and red fruit consumption were positively associated with plasma polyphenols, and were negatively associated with serum malondialdehyde (MDA). Red fruits consumption was particularly positively associated with the ferric reducing ability of plasma (FRAP). Orange-yellow vegetables and fruits consumption were positively associated with plasma vitamin A. However, different colors of vegetables and fruits consumption were not associated with plasma vitamin E and serum protein carbonyl (PC). In conclusion, the consumption of vegetables and fruits in healthy adults was similar to the results of 2005~2008 Nutrition and Health Survey in Taiwan, and was lower than the recommended daily intake guideline. If it can increase the total intake of vegetables and fruits in the daily diet, and including green vegetables, red fruits, orange-yellow vegetables and fruits that might have positive effect against oxidative stress.
    關聯: 88頁
    描述: 第一章 前言 1
    第二章 文獻回顧 2
    第一節 蔬果的攝取與疾病之相關性 2
    第二節 自由基與氧化壓力 3
    第三節 蔬果中的抗氧化物 10
    第三章 研究動機與目的 19
    第四章 研究方法 20
    第一節 研究架構 20
    第二節 試驗設計 21
    第三節 實施程序流程 22
    第四節 招募受試者 23
    第五節 綜合問卷 24
    第六節 檢體分析 29
    第七節 統計方法 36
    第五章 結果 37
    第一節 受試者的基本資料 37
    第二節 蔬果頻率問卷評估 40
    第三節 總蔬果的攝取量與生活型態的關係 52
    第四節 蔬果攝取量與氧化壓力的關係 54
    第六章 討論 63
    第一節 蔬果多樣化與蔬果攝取量的關係 63
    第二節 總蔬果的攝取量與氧化壓力的關係 64
    第三節 不同顏色蔬果的攝取量與氧化壓力的關係 65
    第七章 結論 66
    第八章 研究限制與建議 67
    第九章 參考文獻 68
    第十章 附錄 74


    1993 ~1996 國民營養健康狀況變遷調查,訓練手冊
    http://srda.sinica.edu.tw/Webpages/nahsit/1993/book/1-09.htm (2010年4月 2日上網)

    台灣癌症基金會 http://www.canceraway.org.tw/ (2010年4月2日上網)

    吳幸娟、葉乃華、潘文涵 (2010) 台灣成年與老年人營養素及食物攝取來源之變遷趨勢:由 NASHIT 1993-1996至2005-2008 摘要

    金惠民、王秀媛、邵蘊萍、蘇秀悅、周秋燕、陳幸宜、呂美滿 (1998)台灣常見食品營養圖鑑。行政院衛生署,台北市。

    林雅玲 (2004) 探討台灣鄉土蔬菜 - 紅甘藷葉中多酚類之生體可利用率及對人體抗氧化狀態之影響。台北醫學大學保健營養學研究所碩士論文。

    林薇、劉貴雲、杭極敏、高美丁、黃巧燕 (2005) 每日飲食指南-成人均衡飲食建議量參考手冊。行政院衛生署,台北市。

    食品工業發展研究所與屏東科技大學 (1998) 台灣地區食品營養成分資料庫,pp.94-113,行政院衛生署,台北市。

    湯淑貞 (2000) 台灣紅色鄉土蔬菜萃取物之抗氧化效力研究。中國文化大學生活應用科學研究所碩士論文。

    黃萾芬 (2007) 紅甘藷葉介入對經中強度運動後體內氧化壓力、細胞激素及熱休克蛋白表現之影響。台北醫學大學保健營養學研究所碩士論文。

    5 A DAY message, Department of Health
    http://www.dh.gov.uk/en/Publichealth/Healthimprovement/FiveADay/FiveADaygeneralinformation/DH_4001494 (accessed 10 June 2009)

    Akbulut H, Akbulut KG, Icli F, Buyukcelik A. (2003) Daily variations of plasma malondialdehyde levels in patients with early breast cancer. Cancer Detect Prev. 27:122-126.

    Bakan E, Taysi S, Polat MF, Dalga S, Umudum Z, Bakan N, Gumus M. (2002) Nitric oxide levels and lipid peroxidation in plasma of patients with gastric cancer. Jpn J Clin Oncol. 32:162-166.

    Bashan N, Kovsan J, Kachko I, Ovadia H, Rudich A. (2009) Positive and negative regulation of insulin signaling by reactive oxygen and nitrogen species. Physiol Rev. 89:27-71.

    Beal MF. (2002) Oxidatively modified proteins in aging and disease. Free Radic Biol Med. 32:797-803.

    Benetou V, Orfanos P, Lagiou P, Trichopoulos D, Boffetta P, Trichopoulou A. (2008) Vegetables and fruits in relation to cancer risk: evidence from the Greek EPIC cohort study. Cancer Epidemiol Biomarkers Prev. 17:387-392.

    Benzie IF, Strain JJ. (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem. 239:70-76.

    Bors W, Heller W, Michel C, Saran M. (1990) Methods Enzymol. 186: 343-355.

    Brat P, Georgé S, Bellamy A, Du Chaffaut L, Scalbert A, Mennen L, Arnault N, Amiot MJ. (2006) Daily polyphenol intake in France from fruit and vegetables. J Nutr. 136:2368-2373.

    Campbell DR, Gross MD, Martini MC, Grandits GA, Slavin JL, Potter JD. (1994) Plasma carotenoids as biomarkers of vegetable and fruit intake. Cancer Epidemiol Biomarkers Prev. 3:493-500.

    Dalle-Donne I, Giustarini D, Colombo R, Rossi R, Milzani A. (2003) Protein carbonylation in human diseases. Trends Mol Med. 9:169-176.

    Dauchet L, Péneau S, Bertrais S, Vergnaud AC, Estaquio C, Kesse-Guyot E, Czernichow S, Favier A, Faure H, Galan P, Hercberg S. (2008) Relationships between different types of fruit and vegetable consumption and serum concentrations of antioxidant vitamins. Br J Nutr. 100:633-641.

    Dizdaroglu M, Jaruga P, Birincioglu M, Rodriguez H. (2002) Free radicalinduced damage to DNA: mechanisms and measurement. Free Radic Biol Med. 32:1102-1115.

    Grattagliano I, Vendemiale G, Boscia F, Micelli-Ferrari T, Cardia L, Altomare E. (1998) Oxidative retinal products and ocular damages in diabetic patients. Free Radic Biol Med. 25:369-372.

    Heber D, Bowerman S. (2001) Applying science to changing dietary patterns. J Nutr. 131:3078S-3081S.

    Hensley K, Robinson KA, Gabbita SP, Salsman S, Floyd RA. (2000) Reactive oxygen species, cell signaling, and cell injury. Free Radic Biol Med. 28:1456-1462.

    Henning SM, Seeram NP, Zhang Y, Li L, Gao K, Lee RP, Wang DC, Zerlin A, Karp H, Thames G, Kotlerman J, Li Z, Heber D. (2010) Strawberry consumption is associated with increased antioxidant capacity in serum. J Med Food.13:116-122.

    Higdon JV, Delage B, Williams DE, Dashwood RH. (2007) Cruciferous vegetables and human cancer risk: epidemiologic evidence and mechanistic basis. Pharmacol Res. 55:224-236.

    John JH, Ziebland S, Yudkin P, Roe Ls, Neil HA. (2002) Effects of fruit and vegetable consumption on plasma antioxidant concentrations and blood pressure: a randomized controlled trial. Lancet. 359:1969-1974.

    Kolanjiappan K, Manoharan S, Kayalvizhi M. (2002) Measurement of erythrocyte lipids, lipid peroxidation, antioxidants and osmotic fragility in cervical cancer patients. Clin Chim Acta. 326:143-149.

    Lako J, Trenerry VC, Wahlqvist M, Wattanapenpaiboon N, Sotheeswaran S, Premier R. (2007) Phytochemical flavonols, carotenoids and the antioxidant properties of a wide selection of Fijian fruit, vegetables and other readily available foods. Food Chem. 101:1727-1741.

    Liu RH. (2004) Potential synergy of phytochemicals in cancer prevention: mechanism of action. J Nutr. 134:3479S-3485S.

    Liu S, Manson JE, Lee IM, Cole SR, Hennekens CH, Willett WC, Buring JE. (2000) Fruit and vegetable intake and risk of cardiovascular disease: the Women's Health Study. Am J Clin Nutr. 72:899-900.

    Manach C, Scalbert A, Morand C, Rémésy C, Jiménez L (2004) Polyphenols: food sources and bioavailability. Am J Clin Nutr. 79:727-747.

    Mayne ST. (2003) Antioxidant nutrients and chronic disease: use of biomarkers of exposure and oxidative stress status in epidemiologic research. J Nutr. 133:933S-940S.

    McCrory MA, Fuss PJ, McCallum JE, Yao M, Vinken AG, Hays NP, Roberts SB. (1999) Dietary variety within food groups: association with energy intake and body fatness in men and women. Am J Clin Nutr. 69:440-447.

    Mennen LI, Sapinho D, de Bree A, Arnault N, Bertrais S, Galan P, Hercberg S. (2004) Consumption of foods rich in flavonoids is related to a decreased cardiovascular risk in apparently healthy French women. J Nutr. 134:923-926.

    Niki E, Noguchi N, Tsuchihashi H, Gotoh N. (1995) Interaction among vitamin C, vitamin E, and beta-carotene. Am J Clin Nutr. 62:1322S-1326S.

    Ovaskainen ML, Törrönen R, Koponen JM, Sinkko H, Hellström J, Reinivuo H, Mattila P. Dietary intake and major food sources of polyphenols in Finnish adults. (2008) J Nutr. 138:562-566.

    Paiva SA, Russell RM. (1999) Beta-carotene and other carotenoids as antioxidants. J Am Coll Nutr. 18:426-433.

    Pietta PG. (2000) Flavonoids as antioxidants. J Nat Prod. 63:1035-1042.

    Powolny AA, Singh SV (2008) Multitargeted prevention and therapy of cancer by diallyl trisulfide and related Allium vegetable-derived organosulfur compounds. Cancer Lett. 269:305-314.

    Radhika G, Sudha V, Mohan Sathya R, Ganesan A, Mohan V. (2008) Association of fruit and vegetable intake with cardiovascular risk factors in urban south Indians. Br J Nutr. 99:398-405.

    Rice-Evans CA. (2001) Flavonoid antioxidants. Curr Med Chem. 8:797-807.

    Sies H. (1985) Oxidative stress. Introductory remarks. In: Oxidative Stress (Sies, H., ed.), pp.1-8. Academic Press, London, U.K.

    Spencer JP, Abd El Mohsen MM, Minihane AM, Mathers JC. (2008) Biomarkers of the intake of dietary polyphenols: strengths, limitations and application in nutrition research. Br J Nutr. 99:12-22.

    Singleton VL, Orthofer R and Lamuela-Raventos RM. (1998) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Method in Enzy. 299:152-178.

    Tamer L, Sucu N, Polat G, Ercan B, Aytacoglu B, Yücebilgiç G, Unlü A, Dikmengil M, Atik U. (2002) Decreased serum total antioxidant status and erythrocyte-reduced glutathione levels are associated with increased serum alondialdehyde in atherosclerotic patients. Arch Med Res. 33:257-260.

    Tamer L, Sucu N, Polat G, Ercan B, Aytacoglu B, Yücebilgiç G, Unlü A, Dikmengil M, Atik U. (2002) Decreased serum total antioxidant status and erythrocyte-reduced glutathione levels are associated with increased serum alondialdehyde in atherosclerotic patients. Arch Med Res. 33:257-260.

    Taylor, A. (1993) Cataract: relationship between nutrition and oxidation. J. Am. Coll. Nutr. 12:138-146.

    U.S. Department of Agriculture. (2007) USDA Database for the Flavonoid Content of Selected Foods, Release 2.1.

    Talwar D, Ha TK, Cooney J, Brownlee C, O'Reilly DS. (1998) A routine method for the simultaneous measurement of retinol, alpha-tocopherol and five carotenoids in human plasma by reverse phase HPLC. Clin Chim Acta. 270:85-100.

    Thompson HJ, Heimendinger J, Diker A, O'Neill C, Haegele A, Meinecke B, Wolfe P, Sedlacek S, Zhu Z and Jiang W. (2006) Dietary botanical diversity affects the reduction of oxidative biomarkers in women due to high vegetable and fruit intake. J Nutr. 136:2207-2212.

    Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. (2006) Free radicals, metals and antioxidants in oxidative stress-induced cancer.Chem Biol Interact. 160:1-40.

    Walter MF, Jacob RF, Jeff ers B, Ghadanfar MM, Preston GM, Buch J and Mason RP (2004) Serum levels of thiobarbituric acid reactive substances predict cardiovascular events in patients with stable coronary artery disease. J Am Coll Cardiol. 44:1996-2002.

    Wang J, Shaw N. (2003) Dietary variety and its effect on nutrient adequacy of the Taiwanese diet in the nutritional survey of NAHSIT I 1993-1996. Nutr Sci J. 28:52-64.

    WCRF (2007) The Second Expert Report: Food, Nutrition, Physical Activity and the Prevention of Cancer: A Global Perspective. WCRF/AICR. Washington DC.

    Willcox JK, Ash SL, Catignani GL. (2004) Antioxidants and prevention of chronic disease. Crit Rev Food Sci Nutr. 44:275-295.

    World Health Organisation (2002) The World Health Report 2002: Reducing Risks, Promoting Healthy Life. World Health Organization, Geneva.

    World Health Organization (2003) Diet, nutrition and the prevention of chronic diseases. Report of a Joint FAO/WHO Expert Consultation. WHO Technical Report Series, No. 916. World Health Organization. Geneva.

    Yeh CC, Hsieh LL, Tang R, Chang-Chieh CR, Sung FC. (2003) Risk factors for colorectal cancer in Taiwan: a hospital-based case-control study. J Formos Med Assoc. 102:305-312.
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