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    題名: Antigen Coupled with Lewis-x Trisaccharides Elicits Potent Immune Response in mice
    作者: 蔡宗憲
    Hsu SC;Tsai TH;Kawasaki H;Chen CH;Plunkett B;Lee RT;Huang SK
    貢獻者: 醫學系皮膚學科
    日期: 2007
    上傳時間: 2009-08-24 11:45:53 (UTC+8)
    摘要: BACKGROUND: Glycoproteins containing Lewis-x (Le(x)) trisaccharides are often associated with the host's adaptive T(H)2-type immunity, but the mechanisms underlying the T(H)2-biased response are at present unclear.

    OBJECTIVE: The modulatory effect of Le(x) or its glycoconjugates on IgE/T(H)2 responses was investigated.

    METHODS: The levels of serum antibodies and cytokines were analyzed by means of ELISA, RT-PCR, or both.

    RESULTS: In C3H mice Le(x) coupled with BSA (Le(x)-BSA) elicited higher levels of specific IgE and IgG1, but not IgG2a, which were associated with increased levels of splenic T(H)2 cytokines when compared with those seen in BSA-sensitized mice. In BALB/c mice sensitized with Le(x)-BSA or Le(x) mixed with ovalbumin, significantly increased levels of specific IgE and IgG2a antibodies were found concomitant with reduced levels of serum IL-12p70. These effects were attenuated in IL-12-deficient BALB/c mice. Le(x) and an isomer, Le(y), but not other isomers, inhibited the production of LPS-induced IL-12p70, associated with a significant reduction of nuclear NF-kappaB, in bone marrow-derived dendritic cells from BALB/c mice, suggesting that Le(x)-induced suppression of IL-12p70 results in an enhanced T(H)2 response. The addition of mannan, a known ligand for dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin, abrogated the suppressive effect of Le(x) trisaccharides.

    CONCLUSION: These results provide evidence for a potential role of Le(x) trisaccharides in shaping the immune responses through, at least in part, its suppressive effect on IL-12p70 production. Considering the relative ubiquity of glycoproteins with Le(x) or similar oligosaccharides, including plant-derived (or food-derived) allergens, these findings might have a broad implication.

    CLINICAL IMPLICATIONS: The adjuvant activity of Le(x) trisaccharides might aid in vaccine design and might be important in determining the allergenicity of proteins containing this or other similar structures
    關聯: J Allergy Clin Immunol.(119):1522-1528.
    顯示於類別:[皮膚學科] 期刊論文

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