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| 題名: | Coumarin衍生物的抗氧化作用之活性探討 |
| 作者: | 周裕翔 |
| 貢獻者: | 醫學科學研究所 |
| 日期: | 2010 |
| 上傳時間: | 2010-08-31 10:44:02 (UTC+8) |
| 摘要: | 在之前的研究中我們確定了baicalein會刺激B16F10小鼠黑色素瘤細胞株產生superoxide及hydroxyl radical，且認為此reactive oxygen species (ROS)的生成過程與arachidonic acid (AA)的代謝路徑有關，同時baicalein也造成了細胞存活率下降和細胞凋亡。本次研究我們用electron spin resonance (ESR)直接分析不同的系統，來偵測coumarin 衍生物的抗氧化活性。第一個系統是使用DPPH，分析結果發現各種coumarin 衍生物皆有抗氧化的活性；第二個系統是xanthine oxidase / hypoxanthine系統，直接偵測superoxide的強度，結果發現各種coumarin 衍生物也都有抗氧化的活性；第三個系統是直接分析B16F10老鼠黑色素瘤腫瘤細胞受到baicalein刺激後所釋放之hydroxyl radical，結果發現在coumarin 衍生物中的Mei-174、Mei-215、Mei-228、Mei-234也有抗氧化的活性。我們也使用MTT assay來觀察其對於B16F10小鼠黑色素瘤細胞是否可以減少baicalein所造成的細胞存活率下降，結果發現給予各種coumarin 衍生物皆沒有明顯的影響。各種coumarin 衍生物在不同系統的抗氧化程度可供日後繼續探討其機轉的參考。
In our previous study, we demonstrated that baicalein induced superoxide and hydroxyl radical formation in B16F10 mouse melanoma cell line. We also found the reactive oxygen species (ROS) production is related to the arachidonic acid (AA) metabolism way. Simultaneously baicalein caused reduction of cellular viability and cell apoptosis. In this study, we use electron spin resonance (ESR) to analyze different systems, investigating the antioxidant activity of coumarin derivatives. System I using DPPH and found that coumarin derivatives all have the antioxidant activity. System II is xanthine oxidase / hypoxanthine system, directly investigating the superoxide intensity, and we found that coumarin derivatives also all have the antioxidant activity. System III directly analyze baicalein induced hydroxyl radical formation in B16F10 mouse melanoma, we found that coumarin derivatives Mei-174、Mei-215、Mei-228 and Mei-234 have the antioxidant activity. We also use coumarin derivatives to observe whether they can reduce the reduction of cellular viability effect to baicalein in B16F10 cell by MTT assay, the data show that coumarin derivatives have no obvious effect on cellular
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viability. The antioxidant activity of coumarin derivatives in different systems can refer to the future study of their mechanism. |
| 關聯: | 57頁 |
| 描述: | 中文摘要…………………………………………… 2
英文摘要…………………………………………… 3
縮寫表……………………………………………… 5
壹、緒論…………………………………………… 6
貳、材料與方法
實驗材料……………………………………… 19
實驗方法……………………………………… 23
参、結果…………………………………………… 27
肆、討論…………………………………………… 30
伍、結論與展望…………………………………… 34
陸、附圖…………………………………………… 35
柒、圖表…………………………………………… 37
捌、參考文獻……………………………………… 47
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