Molecular imaging of enhanced Na+ expression in the liver of total sleep deprived rats by TOF-SIMS

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TMUIR > College of Medicine > Department of Medicine > Department of Biochemistry and Molecular Cell Biology > Periodical Article > Item 987654321/3028

Please use this identifier to cite or link to this item: http://libir.tmu.edu.tw/handle/987654321/3028

Title:	Molecular imaging of enhanced Na+ expression in the liver of total sleep deprived rats by TOF-SIMS
Authors:	麥富德 Chang HM;Chen BJ;Wu UI;Huang YL;Mai FD
Contributors:	醫學系生化學科
Date:	2008
Issue Date:	2009-08-21 16:33:56 (UTC+8)
Abstract:	Sleep disorder is associated with metabolic disturbances, which was related to oxidative stress and subsequently sodium overload. Since liver plays important roles in metabolic regulation, present study is aimed to determine whether hepatic sodium, together with oxidative stress, would significantly alter after total sleep deprivation (TSD). Sodium ion was investigated by time-of-flight secondary ion mass spectrometry (TOF-SIMS). Parameter for oxidative stress was examined by heat shock protein-25 (HSP-25) immunohistochemistry. TOF-SIMS spectrum indicated that hepatic Na+/K+ ratio counting as 82.41 ± 9.5 was obtained in normal rats. Sodium ions were distributed in hepatocytes with several aggregations. However, following TSD, the intensity for Na+/K+ ratio was relatively increased (101.94 ± 6.9) and signals for sodium image were strongly expressed throughout hepatocytes without spatial localization. Quantitative analysis revealed that HSP-25 staining intensity is 1.78 ± 0.27 in TSD rats, which was significantly higher than that of normal ones (0.68 ± 0.15). HSP-25 augmentation suggests that hepatocytes suffer from oxidative stress following TSD. Concerning oxidative stress induced sodium overload would impair metabolic function; enhanced hepatic sodium expression after TSD may be a major cause of TSD relevant metabolic diseases. Keywords: Sodium; Hepatocyte; Sleep deprivation; Oxidative stress; TOF-SIMS; Image analysis
Relation:	Applied Surface Science.(255):1131-1134.
Data Type:	article
Appears in Collections:	[Department of Biochemistry and Molecular Cell Biology] Periodical Article

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