Measurement of the second virial coefficient of DPPC- and DPPG-liposomes by isothermal titration calorimetry

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Title:	Measurement of the second virial coefficient of DPPC- and DPPG-liposomes by isothermal titration calorimetry
Authors:	黃豪銘;劉得任 Lin YM;Liu DZ;Huang HM;Tseng LP
Contributors:	生醫材料暨工程研究所
Date:	2007
Issue Date:	2009-08-20 12:47:04 (UTC+8)
Abstract:	本實驗主要利用高靈敏度恆溫熱微上門地以量測DPPC微脂粒以及DPPG微脂粒之第二維里係數，同時利用第二維里系數說明微脂粒物理穩定度。實驗系統是利用恆溫熱微卡計量測微脂粒懸浮液之稀釋熱，再配合數學模式之演譯計算出微脂粒懸浮液系統之第二維里數b2說明微脂粒粒子間交互作用，另外，我們更進一步利用能量位能井方程式說明微脂粒聚集融合之能量障壁參數b2/B(下標 o)。最後，我們也利用此模式證實，無論是b2或b2/b(下標 o)值以及物理穩定度，DPPC微脂粒都明顯較DPPC微脂粒高。 In this study, we employed a thermodynamic method to obtain information on the second virial coefficient of the interactions between liposomes, and we also determined the stabilities of dipalmitoyl-L-alpha-phosphatidylglycesol (DPPG)-and dipalmitoyl-L-alphha-phosphatidylcho-line (DPPG)-liposomes. In this study, an interaction model based on a hard-sphere interaction potential assumption was employed, and we used high-sensitivity isothermal titration nanocalorimetry (ITC) to measure the diltion heat of liposomal suspension. Information on the second virial coefficient of interactions between liposomes can be derived from the number density of the liposomal suspension. Additionally, we qualitatively demonstrated the repulsive forces of DPPG-and DPPC-liposomes systems. In this work, both the b2 and b2/B(subscript o) levels of DPPG-liposome were greater and had better physical stability than those of DPPG-liposome.
Relation:	J Chin Inst Chem Eng.(38):103-106.
Data Type:	article
Appears in Collections:	[Graduate Institute of Biomedical Materials and Tissue Engineering] Periodical Article

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