Decreased expression of mitochondrial genes in human unfertilized oocytes and arrested embryos.

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TMUIR > College of Medicine > Department of Medicine > Department of Obstetrics and Gynecology > Periodical Article > Item 987654321/16788

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Title:	Decreased expression of mitochondrial genes in human unfertilized oocytes and arrested embryos.
Authors:	曾啟瑞 Hsieh RH;Au HK;Yeh TS;Chang SJ;Cheng YF;Tzeng CR
Contributors:	醫學系婦產學科
Date:	2004
Issue Date:	2009-11-13 10:04:54 (UTC+8)
Abstract:	OBJECTIVE: To evaluate the relationship between mitochondrial gene expression of oocytes/embryos and their fertilizability in unfertilized oocytes, arrested embryos, and tripronucleate zygotes, because both nuclear and cytoplasmic factors contribute to oocyte activation, fertilization, and subsequent development. DESIGN: Prospective laboratory research. SETTING: In vitro fertilization (IVF) laboratory in a university hospital. PATIENT(S): Seventy-five unfertilized oocytes, 45 arrested embryos, and 24 tripronucleate (3PN) embryos from 45 female patients undergoing IVF. INTERVENTION(S): Analysis of mitochondrial gene expression by semiquantitative reverse transcription polymerase chain reaction (RT-PCR). MAIN OUTCOME MEASURE(S): Comparison of the expression levels of mitochondrial genes including ND2, CO I, CO II, ATPase 6, CO III, ND3, ND6, and Cyt b in three groups. RESULT(S): Significantly decreased transcription levels were expressed in unfertilized oocytes and arrested embryos. The average expression levels of the eight determined genes compared with the control (GAPDH) was 4.4 +/- 0.7, 6.4 +/- 1.1, and 13.2 +/- 1.1 in unfertilized oocytes, arrested embryos, and 3PN embryos, respectively. Significantly decreased expressions of the ATPase 6, CO III, and ND3 genes were detected from samples with 4977-bp common deletion in the mitochondrial DNA (mtDNA) compared with the non-deletion group. CONCLUSION(S): The present study is the first report to present globally decreased mitochondrial gene expression levels in human compromised oocytes and embryos. These data support the notion that the down-regulation of mitochondrial RNA by defective oxidative phosphorylation genes possibly affects oocyte quality including fertilization and further embryo development.
Relation:	Fertility and Sterility.(81):912-918.
Data Type:	article
Appears in Collections:	[Department of Obstetrics and Gynecology] Periodical Article

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