Stimultaneous determination of podophyllotoxin;  quercetin and kaempferol in podophyllin by liquid chromatography Tandem Mass Spectrometry

English | 正體中文 | 简体中文 | 全文筆數/總筆數 : 45422/58598 (78%)
造訪人次 : 2530141 線上人數 : 241

RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.

Taipei Medical University Institutional Repository > 藥學院 > 藥學系 > 期刊論文 > Item 987654321/1640

請使用永久網址來引用或連結此文件: http://libir.tmu.edu.tw/handle/987654321/1640

題名:	Stimultaneous determination of podophyllotoxin;quercetin and kaempferol in podophyllin by liquid chromatography Tandem Mass Spectrometry
作者:	鄭幼文;鄭慧文 Lin MC;Lin JH;Chen SK;Cheng YW;Cheng HW
貢獻者:	藥學系
日期:	2008
上傳時間:	2009-08-17 09:56:16 (UTC+8)
摘要:	一種定量之高效液相層析串聯式質譜法被開發，以用於分析鬼臼樹脂(Podophyllin)中成分。使用Cosmosil碳18管柱作層析分離，移動相爲0.25%甲酸－甲醇之混合液進行梯度沖提。經管柱分離後之流出液以2:3之比例，分別進入紫外光可見光檢測器及串聯質譜儀。以子代離子掃瞄鑑定鬼臼樹脂中podophyllotoxin、quercetin及kaempferol之成分，並以多重離子監測模式測定其含量。Podophyllotoxin、quercetin及kaempferol之偵測極限及定量極限分別爲2.40，8.01；2.94，9.87與3.10，10.2 ng/mL。三成分之同日間及異日間試驗相對標準偏差介於0.52-6.01%及2.48-9.88%。平均回收率各爲101.4%、98.3%及98.7%。由實驗結果可知，所開發之高效液相層析串聯式質譜法可同時偵測分析podophyllin中之podophyllotoxin、quercetin及kaempferol成分。 A quantitative high performance liquid chromatography-tandem mass spectrometry (LC/MS/MS) was developed to analyze the constituents of podophyllin. Chromatographic separation was performed on a Cosmosil 5C18-MS (25 cm×4.6 mm I.D., 5 μm) reverse phase column using a gradient of mobile phase (0.25% formic acid-methanol). The column effluent was split 2:3 into the photodiode detector and tandem mass spectrometer. Podophyllotoxin, quercetin and kaempferol in podophyllin were identified by daughter ion scan mode and then determined their contents by multiple-reaction monitoring (MRM) mode. The limits of detection and quantitation for podophyllotoxin, quercetin and kaempferol were 2.40, 8.01; 2.94, 9.87 and 3.10, 10.2 ng/mL, respectively. The relative standard deviations of intraday and interday analyses for podophyllotoxin, quercetin and kaempferol ranged from 0.52-6.01% and 2.48-9.88%, respectively. The mean recoveries for podophyllotoxin, quercetin and kaempferol were 101.4%, 98.3% and 98.7%, respectively. The developed LC/MS/MS method was suitable for the simultaneous determinations of podophyllotoxin, quercetin and kaempferol in podophyllin.
關聯:	Journal of Food and Drug analysis.(16):29-40.
資料類型:	article
顯示於類別:	[藥學系] 期刊論文

文件中的檔案:

檔案	描述	大小	格式	瀏覽次數
219.pdf		1076Kb	Adobe PDF	335	檢視/開啟
全文.txt		0Kb	Text	109	檢視/開啟

在TMUIR中所有的資料項目都受到原著作權保護.

TAIR相關文章

著作權聲明 Copyright Notice

本平台之數位內容為臺北醫學大學所收錄之機構典藏，包含體系內各式學術著作及學術產出。秉持開放取用的精神，提供使用者進行資料檢索、下載與取用，惟仍請適度、合理地於合法範圍內使用本平台之內容，以尊重著作權人之權益。商業上之利用，請先取得著作權人之授權。
The digital content on this platform is part of the Taipei Medical University Institutional Repository, featuring various academic works and outputs from the institution. It offers free access to academic research and public education for non-commercial use. Please use the content appropriately and within legal boundaries to respect copyright owners' rights. For commercial use, please obtain prior authorization from the copyright owner.
瀏覽或使用本平台，視同使用者已完全接受並瞭解聲明中所有規範、中華民國相關法規、一切國際網路規定及使用慣例，並不得為任何不法目的使用TMUIR。
By utilising the platform, users are deemed to have fully accepted and understood all the regulations set out in the statement, relevant laws of the Republic of China, all international internet regulations, and usage conventions. Furthermore, users must not use TMUIR for any illegal purposes.
本平台盡力防止侵害著作權人之權益。若發現本平台之數位內容有侵害著作權人權益情事者，煩請權利人通知本平台維護人員([email protected])，將立即採取移除該數位著作等補救措施。
TMUIR is made to protect the interests of copyright owners. If you believe that any material on the website infringes copyright, please contact our staff([email protected]). We will remove the work from the repository.

資料載入中.....