Ethanol enhanced in vivo gene delivery with non-ionic polymeric micelles inhalation

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Taipei Medical University Institutional Repository > 藥學院 > 藥學系 > 期刊論文 > Item 987654321/1590

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題名:	Ethanol enhanced in vivo gene delivery with non-ionic polymeric micelles inhalation
作者:	廖嘉鴻 Chao YC;Chang SF;Lu SC;Hwang TC;Hsieh WH;Liaw J
貢獻者:	藥學系
日期:	2007
上傳時間:	2009-08-17 09:55:48 (UTC+8)
摘要:	Modifications of both carriers and host barriers have been investigated for efficient inhalation gene delivery to lung. Here we used a biocompatible, non-ionic poly(ethyleneoxide)-poly(propyleneoxide)-poly(ethyleneoxide) (PEO-PPO-PEO) polymeric micelles (PM) as a carrier and combined it with ethanol to enhance membrane penetration of delivered DNA. The inhalation delivery with six 100 microg doses of pCMV-Lac Z with PM co-formulated with 10%-40% ethanol to nude mice in 2 days at 8 h interval was performed. The beta-galatosidase (beta-Gal) activity was assessed using chlorophenol red-beta-d galactopyranoside (CPRG) and X-gal staining for quantitative and qualitative analysis in tissues. The results showed that beta-Gal activity was significantly increased by 38% in lung around bronchioles when inhalation with PM and 10% ethanol was given. The 10% ethanol also increased the intracellular apparent permeability by 42% in stomach and by 141% in intestine at 48 h after the first dosage of delivery. Also delivery of DNA encoding a functional human cystic fibrosis transmembrane protein (CFTR) using the same inhalation delivery method co-formulated with 10% ethanol, an increased expression of CFTR in lung was detected by immunostaining. We concluded that 10% ethanol co-formulated with the PM system could enhance inhaled gene delivery to airway and gastrointestinal (GI) tract.
關聯:	Journal of Controlled Release.(118):105-117.
資料類型:	article
顯示於類別:	[藥學系] 期刊論文

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