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    請使用永久網址來引用或連結此文件: http://libir.tmu.edu.tw/handle/987654321/15136


    題名: Development of Probe-Based Ultraweak Chemiluminescence Technique for the Detection of a Panel of Four Oxygen-Derived Free Radicals and Their Applications in the Assessment of Radical-Scavenging Abilities of Extracts and Purified Compounds from Food and He
    作者: 邱仲峰
    Chen CW;Chiou JF;Tsai CH;Shu CW;Lin MH;Liu TZ;Tsai LY
    貢獻者: 醫學系放射線科
    日期: 2006
    上傳時間: 2009-11-03 15:52:39 (UTC+8)
    摘要: We report here the development of a probe-based ultraweak chemiluminescence (uwCL) method capable of detecting a panel of four oxygen-derived free radicals (ODFRs) including superoxide (O2-), hydrogen peroxide (H2O2), hydroxyl radical (*OH), and peroxyl radical (ROO*) using different probes specific for these radicals performed by the same uwCL analyzer. The selected radical-generating systems and their corresponding uwCL-probing emitters were validated. These ODFR-detecting systems were subsequently utilized by us to assess the radical-scavenging ability (RSA) of a variety of extracts and purified constituents derived from foods and herbal preparations. Our approach for assessing RSA for these constituents is based on the suppression of uwCL generated by each ODFR, and the degrees of inhibition have been shown to be dose-dependent. For this reason, the estimation of IC50 for each testing compound can be obtained from the curve constructed based on the percent of inhibitions of uwCL versus the concentrations of the compound tested. To illustrate the practical applications of our devised methodology, data for comparative studies of RSA activities of fermented extracts of Cordeceps sinensis, purified methylgallate isolated from Toona sinesis, resveratrol purified from grape seeds, plus epimedin C from the aerial part of the Epimedium plant (yinyanghuo) are to be presented
    關聯: J Agric Food Chem.(54):9297-9302.
    資料類型: article
    顯示於類別:[放射線學科] 期刊論文

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