Expression and characterization of a brain-specific protein kinase BSK146 from zebrafish

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TMUIR > College of Medicine > Department of Medicine > Department of Biochemistry and Molecular Cell Biology > Periodical Article > Item 987654321/13772

Please use this identifier to cite or link to this item: http://libir.tmu.edu.tw/handle/987654321/13772

Title:	Expression and characterization of a brain-specific protein kinase BSK146 from zebrafish
Authors:	周志銘 Chou CM;Chen YC;Lee MT;Chen GD;Lu IC;Chen ST;Huang CJ
Contributors:	醫學系生化學科
Date:	2006
Issue Date:	2009-10-28 13:55:23 (UTC+8)
Abstract:	We have previously identified a novel protein kinase, pkl46, in the brain of Tetraodon. In the present study, we cloned the homologous protein kinase gene encoding a protein of 385 amino acid residues from zebrafish. The overall amino acid sequence and the kinase domain of zebrafish BSK146 shows 48% and 69% identity to that of rat sbk, a SH3-containing serine/threonine protein kinase. By whole-mount in situ hybridization and RT-PCR, the expression of bsk 146 mRNA was mainly in the brain. To explore the in vivo function of BSK146 during zebrafish development, we used morpholino knockdown approach and found that BSK146 morphants displayed enlarged hindbrain ventricle and smaller eyes. Whole-mount in situ hybridization was further performed to analyze the brain defects in BSK146-MO-injected embryos. The expression of brain-specific markers, such as otx2, pax2.1, and krox20, was found normal in morphant embryos at 24 hpf, while expression of pax2.1 exerted changes in midbrain-hindbrain boundary and hindbrain in morphant embryos at 48 hpf. These data suggest that BSK146 may play an important role in later ventricle expansion in zebrafish brain development. Although the recombinant BSK146 protein produced in insect cells was active and could phosphorylate both histone HI and histone 2B, the endogenous substrate of BSK146 in the embryonic brain of zebrafish is not clear at the present time and needs further investigation.
Relation:	Biochemical and biophysical research Communications.(340):767-775.
Data Type:	article
Appears in Collections:	[Department of Biochemistry and Molecular Cell Biology] Periodical Article

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