Detection of apoptosis and necrosis in normal human lung cells using 1H NMR spectroscopy

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TMUIR > College of Medicine > Department of Medicine > Department of Biochemistry and Molecular Cell Biology > Periodical Article > Item 987654321/13737

Please use this identifier to cite or link to this item: http://libir.tmu.edu.tw/handle/987654321/13737

Title:	Detection of apoptosis and necrosis in normal human lung cells using 1H NMR spectroscopy
Authors:	施純明;羅翠勻;陳建志 Shih CM;Ko WC;Yang LY;Lin CJ;Wu JS;Lo TY;Wang SH;Chen CT
Contributors:	醫學系生化學科
Date:	2005
Issue Date:	2009-10-28 13:54:22 (UTC+8)
Abstract:	This study aimed to detect apoptosis and necrosis in MRC-5, a normal human lung cell line, by using noninvasive proton nuclear magnetic resonance (1H NMR). Live MRC-5 cells were processed first for 1H NMR spectroscopy; subsequently their types and the percentage of cell death were assessed on a flow cytometer. Cadmium (Cd) and mercury (Hg) induced apoptosis and necrosis in MRC-5 cells, respectively, as revealed by phosphatidylserine externalization on a flow cytometer. The spectral intensity ratio of methylene (CH2) resonance (at 1.3 ppm) to methyl (CH3) resonance (at 0.9 ppm) was directly proportional to the percentage of apoptosis and strongly and positively correlated with PI staining after Cd treatment (r2 = 0.9868, P < 0.01). In contrast, this ratio only increased slightly within 2-h Hg treatment, and longer Hg exposure failed to produce further increase. Following 2-h Hg exposure, the spectral intensity of choline resonance (at 3.2 ppm) was abolished, but this phenomenon was absent in Cd-induced apoptosis. These findings together demonstrate that 1H NMR is a novel tool with a quantitative potential to distinguish apoptosis from necrosis as early as the onset of cell death in normal human lung cells.
Relation:	Annals of the New York Academy of Science.(1042):488-496.
Data Type:	article
Appears in Collections:	[Department of Biochemistry and Molecular Cell Biology] Periodical Article

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