JAK1 N-terminus binds to conserved Box1 and Box2 motifs of cytokine receptor common beta subunit but signal activation requires JAK1 C-terminus

English | 正體中文 | 简体中文 | 全文筆數/總筆數 : 44690/57798 (77%)
造訪人次 : 1626171 線上人數 : 72

RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.

Taipei Medical University Institutional Repository > 醫學院 > 醫學科學研究所 > 期刊論文 > Item 987654321/10307

請使用永久網址來引用或連結此文件: http://libir.tmu.edu.tw/handle/987654321/10307

題名:	JAK1 N-terminus binds to conserved Box1 and Box2 motifs of cytokine receptor common beta subunit but signal activation requires JAK1 C-terminus
作者:	黃惠美 Huang HM;Lee YL;Chang TW
貢獻者:	醫學科學研究所
日期:	2006
上傳時間:	2009-10-12 15:32:12 (UTC+8)
摘要:	The human interleukin-3 receptor (hIL-3R) consists of a unique alpha subunit (hIL-3Ralpha) and a common beta subunit (betac). Binding of IL-3 to IL-3R activates Janus kinases JAK1 and JAK2. Our previously study showed that JAK2 and JAK1 were constitutively associated with the hIL-3Ralpha and betac subunits, respectively. In this study, we further demonstrate that JAK2 binds to the intracellular domain of hIL-3Ralpha and JAK1 binds to the Box 1 and Box 2 motifs of betac using GST-hIL-3R fusion proteins in pull-down assays. JAK1 mutational analysis revealed that its JH7-3 domains bound directly to the Box 1 and Box 2 motifs of betac. We further examined the role of JAK1 JH7-3 domains in JAK1 and JAK2-mediated signaling using the CDJAKs fusion proteins, which consisted of a CD16 extracellular domain, a CD7 transmembrane domain, and either JAK1 (CDJAK1), JAK2 (CDJAK2), or JAK1-JH7-3 domains (CDJAK1-JH7-3) as intracellular domains. Anti-CD16 antibody crosslinking of wild type fusion proteins CDJAK1 with CDJAK2 could mimic IL-3 signaling, however, the crosslinking of fusion proteins CDJAK1-JH7-3 with CDJAK2 failed to activate downstream proteins. These results suggest that the JAK1-JH7-3 domains are required for betac interaction and abolish wild type JAK1 and JAK2-mediated signaling.
關聯:	J. Cell Biochem.(99):1078-1084.
顯示於類別:	[醫學科學研究所] 期刊論文

文件中的檔案:

檔案	大小	格式	瀏覽次數
37-2.pdf	147Kb	Adobe PDF	78	檢視/開啟
JAK1 N-terminus binds to conserved Box1 and Box2 motifs of cytokine receptor common beta subunit but signal activation requires JAK1 C-terminus.doc	25Kb	Microsoft Word	101	檢視/開啟
JAK1 N-terminus binds to conserved Box1 and Box2 motifs of cytokine receptor common beta subunit but signal activation requires JAK1 C-terminus.pdf	32Kb	Adobe PDF	70	檢視/開啟
JAK1 N-terminus binds to conserved Box1 and Box2 motifs of cytokine receptor common beta subunit but signal activation requires JAK1 C-terminus.ps	123Kb	Postscript	84	檢視/開啟

在TMUIR中所有的資料項目都受到原著作權保護.

TAIR相關文章

著作權聲明 Copyright Notice

本平台之數位內容為臺北醫學大學所收錄之機構典藏，包含體系內各式學術著作及學術產出。秉持開放取用的精神，提供使用者進行資料檢索、下載與取用，惟仍請適度、合理地於合法範圍內使用本平台之內容，以尊重著作權人之權益。商業上之利用，請先取得著作權人之授權。
The digital content on this platform is part of the Taipei Medical University Institutional Repository, featuring various academic works and outputs from the institution. It offers free access to academic research and public education for non-commercial use. Please use the content appropriately and within legal boundaries to respect copyright owners' rights. For commercial use, please obtain prior authorization from the copyright owner.
瀏覽或使用本平台，視同使用者已完全接受並瞭解聲明中所有規範、中華民國相關法規、一切國際網路規定及使用慣例，並不得為任何不法目的使用TMUIR。
By utilising the platform, users are deemed to have fully accepted and understood all the regulations set out in the statement, relevant laws of the Republic of China, all international internet regulations, and usage conventions. Furthermore, users must not use TMUIR for any illegal purposes.
本平台盡力防止侵害著作權人之權益。若發現本平台之數位內容有侵害著作權人權益情事者，煩請權利人通知本平台維護人員([email protected])，將立即採取移除該數位著作等補救措施。
TMUIR is made to protect the interests of copyright owners. If you believe that any material on the website infringes copyright, please contact our staff([email protected]). We will remove the work from the repository.

資料載入中.....